Thpo deficiency broadly impairs organelle function and turnover. (A-C) Principal component analysis of RNA sequence on Thpo^+/+ (wt), Thpo^−/− (ko), Thpo^+/+ Ro (wtr), and Thpo^−/− Ro (kor) HSCs. Heat map analysis (B) and Venn diagram (C) for differentially expressed genes in Thpo^+/+ (wt), Thpo^−/− (ko), Thpo^+/+ Ro (wtr), and Thpo^−/− Ro (kor) HSCs. (D) Gene set enrichment analysis (GSEA) analysis of HSC- and progenitor-related gene sets in Thpo^+/+ and Thpo^−/− HSCs. (E) GSEA analysis for enrichment in HSC-related gene set for Thpo^−/− and REST (Thpo^+/+, Thpo^+/+ Ro, and Thpo^−/− Ro HSCs). (F) GSEA analysis of apoptosis-related gene set for Thpo^+/+, Thpo^−/−, and REST HSCs. (G) Table and GSEA plots showing enrichment in ribosome-associated gene sets upregulated in REST compared with Thpo^−/− HSCs. (H) Mean fluorescence intensity (MFI) of OPP incorporation in Thpo^+/+, Thpo^−/−, and Thpo^−/− Ro HSCs (n = 5). (I) Analysis of autophagy-related gene set enrichment in Thpo^−/− and REST HSCs. (J) Representative plots and MFI of Cyto-ID staining in Thpo^+/+, Thpo^fl/fl;AlbCre^+/−, and Thpo^−/− HSCs (n = 5). (K) Representative plot and MFI of Cyto-ID staining in Thpo^−/− and Thpo^−/− Ro HSCs (n = 5). *P < .05, **P < .01, Student t test. FDR, false discovery rate; NOM, nominal; p-val, P value; q-val, q value.