Cxcr4^+/1013 mice show a reduced pDC pool in the spleen, LNs, and thymus. (A) Representative dot plots show the gating strategy for cDC1 (CD11c^hiCD317⁻CD11b⁻CD8⁺), cDC2 (CD11c^hiCD317⁻CD11b⁺CD8⁻), and pDC (CD11c^loCD317⁺B220⁺) identification in the spleens from Cxcr4^+/1013 (+/1013) and littermate Cxcr4^+/+ (WT) mice. The percentages of cDCs, CD11c^loCD317⁺ cells, and pDCs among singlets and cDC1 and cDC2 among DCs are indicated. (B-C) Spleen cDCs and pDCs frequencies (B) and numbers (C) were determined by flow cytometry (n = 17 mice/group from 7 experiments). (D,G) Representative dot plots showing pDC gating in inguinal LNs (D) and the thymus (G) from +/1013 and WT mice. (E-F) Resident cDC (resDC, CD11c^hiCD317⁻MHCII⁺) and pDC (CD11c^loCD317⁺B220⁺) frequencies (E) and numbers (F) in inguinal LNs from +/1013 and WT mice (n = 12-13 mice/group from 5 experiments). (H-I) cDC (CD11c^hiCD317⁻) and pDC (CD11c^loCD317⁺B220⁺) frequencies (H) and numbers (I) in thymuses from +/1013 and WT mice (n = 7-8 mice/group from 3 experiments). Bar graphs show the mean ± SEM. Mice had a C57BL/6J genetic background. Statistical analysis was performed using the 2-tailed, unpaired Mann-Whitney test. *P < .05, **P < .01, ***P < .001.