OBF1 is required for the maintenance of the GC transcriptional program. (A) Left, average expression (log₂ counts per million, x-axis) and change of expression (log₂-fold change, y-axis) for genes in control (SHC002) and OBF1 knockdown (Obf1sh1) conditions. Differentially expressed genes are color-coded (DEG fold change >1.5, FDR <0.001, logCPM >2): red data points represent genes with higher expression level in Raji cells treated with OBF1-specific shRNA; green data points represent genes with higher expression in control-treated (scrambled shRNA sequence) Raji cells. Right, gene ontology analysis was performed with the genes up- and downregulated in OBF1 knockout vs WT splenic B cells stimulated with anti-CD40/IL4. (B) Heatmaps showing differentially expressed genes involved in GC reaction between Raji cells infected with lentivirus expressing control shRNA (SHC002) and OBF1-specific shRNA. (C) GSEA of relative gene expression in OBF1 depleted vs WT Raji cells against the gene set identified as genes upregulated in plasma cells. NES, normalized enrichment score. (D) Average expression (log₂ counts per million, x-axis) and change of expression (log₂-fold change, y-axis) for genes in WT and ectopically IRF4 expressing Raji cells. Differentially expressed genes are color-coded (DEG fold change ≥1, FDR <0.01, logCPM >2): red data points represent genes with higher expression level in ectopically IRF4-expressing Raji cells, green data points represent genes with higher expression in WT Raji cells. (E) GSEA of relative gene expression in ectopically IRF4-expressing vs WT Raji cells against the differentially expressed genes in OBF1-depleted Raji cells. NES, normalized enrichment score. (F) OBF1 CUT&RUN read densities at 2 individual loci in Raji and SUDHL4 cells. (G) H3K27ac and OBF1 CUT&RUN read densities at 2 individual loci, as indicated, in purified germinal center B cells from human tonsils. (H) GSEA of relative gene expression in OBF1-depleted vs WT Raji cells against the gene set identified as genes associated with favorable prognosis for patients with DLBCL.