Hemophilia A mutations in FVIII and VWD type 2N mutations in VWF-D′D3 decorate the protein-protein interface. (A) Hemophilia A missense mutations in the interface with VWF are shown on FVIII as gray spheres, with prevalent hemophilia A mutations labeled and depicted as black spheres (defined here as >10 cases reported in the European Association for Haemophilia and Allied Disorders F8 variant database.⁵⁰  (B) VWD type 2N mutations, as annotated in Dong et al,²⁵  are shown on VWF-D′D3. Mutations in gray are in the interface with FVIII. Mutations in white are outside the FVIII interface, as annotated by PDB PISA. The calcium ion in VWF-D3 is not shown.