Figure 3.
Sepsis platelets show increased GPVI ectodomain shedding. Assays display the following time points: I: admission day; II: day 5 to 7; and III: day of ICU discharge. (A-B) CLEC-2 (A) and GPVI (B) expression on platelet surface were analyzed by flow cytometry. (C) GPVI expression studied by western blotting using JAQ-1 antibody. Platelet lysis was performed 60 minutes after stimulation with CRP-XL (C) (0.1 µg/mL) or under resting conditions (−). (D) ELISA for soluble GPVI ectodomain (sGPVI) was performed with plasma of sepsis patients and corresponding controls. Graphs show median ± IQR. Differences were analyzed by Kolmogorov-Smirnov test (A) or Kruskal-Wallis test (B,D). *P < .05; **P < .01. AU, arbitrary units; α-GAPDH, α-glyceraldehyde-3-phosphate dehydrogenase.

Sepsis platelets show increased GPVI ectodomain shedding. Assays display the following time points: I: admission day; II: day 5 to 7; and III: day of ICU discharge. (A-B) CLEC-2 (A) and GPVI (B) expression on platelet surface were analyzed by flow cytometry. (C) GPVI expression studied by western blotting using JAQ-1 antibody. Platelet lysis was performed 60 minutes after stimulation with CRP-XL (C) (0.1 µg/mL) or under resting conditions (−). (D) ELISA for soluble GPVI ectodomain (sGPVI) was performed with plasma of sepsis patients and corresponding controls. Graphs show median ± IQR. Differences were analyzed by Kolmogorov-Smirnov test (A) or Kruskal-Wallis test (B,D). *P < .05; **P < .01. AU, arbitrary units; α-GAPDH, α-glyceraldehyde-3-phosphate dehydrogenase.

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