Presence of FLT3-ITD clones at diagnosis and disease progression based on a Genescan-based routine diagnostic assay. Each column represents 1 patient. (A) Presence of FLT3-ITD clones at diagnosis (D) and relapse/refractory disease (R) in patients treated with midostaurin. (B) Presence of FLT3-ITD clones between D and R in 21 intensively treated patients without the addition of midostaurin. Green indicates that the same ITDs were detected at both time points. A change of ITDs between D and R is color-coded in orange (light, loss of ITDs at progression with at least 1 persistent FLT3-ITD between both time points; medium, switch of ITD insertion site or ITD length; dark, gain of ITD at R). Blue indicates that no FLT3-ITDs were detected at R.