Patterns of clonal evolution in relapse patients. Fish plots visualizing patterns of clonal evolution in relapse patients according to the variant allele frequencies at diagnosis, remission, and relapse in patients with persistent FLT3-ITD (relapse occurred after midostaurin maintenance cycle 11; A), change of FLT3-ITD (relapse after cycle 3; B), and loss of FLT3-ITD (relapse after cycle 3; C). Information on treatment and BM blast count is given below each plot. HDAC, high-dose cytarabine; HSCT, hematopoietic stem cell transplantation; NA, not available.