Figure 2.
Global mitochondrial hypomethylation in cfDNA samples. Bisulfite sequencing (BS-Seq) graphical output of mitochondrial DMRs in the cfDNA of patients with SCD. cfDNA from 7 longitudinal pairs (7 SCD-Baseline and 7 SCD-Crisis) of patients with SCD and an HC was extracted and treated with bisulfite, and the whole genome was sequenced for the BS-Seq analysis to analyze the levels of cytosine guanine dinucleotide (CpG) across the samples. DMRs with ≥3 CpGs and a mean methylation difference between groups of samples ≥10% are considered significant. Blue and red lines indicate mean percentage of methylation of SCD-Baseline and SCD-Crisis, respectively, across 7 pairs. The green line represents the percentage of mitochondrial methylation in the HC.

Global mitochondrial hypomethylation in cfDNA samples. Bisulfite sequencing (BS-Seq) graphical output of mitochondrial DMRs in the cfDNA of patients with SCD. cfDNA from 7 longitudinal pairs (7 SCD-Baseline and 7 SCD-Crisis) of patients with SCD and an HC was extracted and treated with bisulfite, and the whole genome was sequenced for the BS-Seq analysis to analyze the levels of cytosine guanine dinucleotide (CpG) across the samples. DMRs with ≥3 CpGs and a mean methylation difference between groups of samples ≥10% are considered significant. Blue and red lines indicate mean percentage of methylation of SCD-Baseline and SCD-Crisis, respectively, across 7 pairs. The green line represents the percentage of mitochondrial methylation in the HC.

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