Sickle cell RBCs retain mitochondria. Confocal imaging of blood cells from a patient who was SCD-Baseline stained with mitochondrial (MTG; green), and nuclear (Hoechst; blue) markers. The stained live cells were plated onto 35-mm No. 1.5 poly-d-lysine–coated glass-bottom Petri dishes, and superresolution imaging was performed on the instant SIM. Note that the majority of cells that stained with MTG are anucleate (Hoechst negative). A 3-dimensional superresolution imaging of this figure showing mitochondrial retention in red cells is shown in supplemental Video 1 containing cells that are stained with mitochondrial markers MTG (green), and TMRM (red), markers that stain inactive (depolorized) and active (polarized) mitochondria respectively, and nuclear marker Hoechst (blue).