PHD2-deficient neutrophils present a distinct signature associated to cytoskeleton dynamics. (A) Schematic overview of the deep-sequencing approach, comparing RNA sequencing data from cKO P2 and cKO P2H2 mice, as well as their respective WT littermates. Apoptosis signatures in both transgenic lines are not changed significantly compared with their WT littermates (B), whereas glycolysis/gluconeogenesis signatures are highly correlated with cKO P2 and cKO P2H2, suggesting an HIF2-independent phenotype (C). (D) cKO P2 and cKO P2H2 mice display a negative correlation, with signatures related to the innate immune response but not chemokine signaling. (E) A number of signatures significantly linked to functioning and structure of the cytoskeleton are found only in cKO P2 vs WT littermate neutrophils. NES, normalized enrichment score.