Overexpression of CYTL1 in CMML monocytes. (A) Volcano plot analysis of differentially methylated regions identified by ERRBS by comparing CMML monocytes with defective (n = 10) and nondefective (n = 9) apoptosis, based on their survival after 24 hours in serum-containing medium (>50% or ≤50%). (B) Gene Ontology (GO) pathway analysis of ERRBS data, identifying MAP3K as the most significant differentially regulated pathway. (C) Volcano plot analysis of differentially expressed genes identified by RNA-seq of samples analyzed in panel A. (D) Healthy donor (HD) monocytes were incubated for 24 hours in culture medium with 10% peripheral blood plasma collected from healthy donors (n = 4) or patients with CMML (n = 19). The fraction of surviving cells (AnV^–/PI^–) was normalized to that of control monocytes in serum-free medium. (E) CYTL1 gene expression measured by RT-qPCR in healthy donor and CMML monocytes, using RPL32 as a housekeeping gene. (F) CYTL1 gene expression analyzed in RNA-seq data from Franzini et al.²⁰  Kruskal-Wallis test, Dunn’s multiple comparison: **P < .01. (G) CYTL1 protein level measured in the peripheral blood plasma of healthy donors and patients with CMML. Error bars are mean ± SEM. Mann-Whitney U test (D-E,G): *P < .05; **P < .01; ****P < .0001.