Cytokine sensitivity and expression in AF10 FP-expressing AMLs. (A) Number of viable cells per 10 000 initially seeded cells at day 5 after cytokine withdrawal is plotted on the y-axis for the indicated AML FPs. (B) Schematic on top shows the format of the coculture experiment in which a Transwell was inserted into a 6-well plate for coculture. Number of viable cells per 10 000 cells at day 3 with MLL-AF9 AML cells cocultured with the same cells on top (left panel) or overlaid with CALM-AF10 or MLL-AF10 AML cells (right panels) is shown. (C) Normalized spot intensity of indicated cytokines is shown in the bar graph for M-CSF, GM-CSF, and IL3 for CALM-AF10 On (gray bars) and Off cells (blue bars) and (D) for iMLL-AF10 On (gray) and Off (blue bars) with indicated P values. *P < .05 calculated using the Student t test.