Inflammasome activation triggers venous thrombosis following IVC ligation. (A-D) WT (n = 16) and caspase-1–deficient mice (n = 17) were subjected to IVC stenosis. Thrombus prevalence (A), thrombus weight (B), and thrombus length (C) were measured at 48 hours after the surgery. Representative images of thrombi are shown in panel D. Lines in dot plots represent medians. ^⋆P < .05; ^⋆⋆P < .01. The χ² method was used in panel A and Mann-Whitney U test was used in panels B and C. (E-G) WT mice (n = 14) and caspase-11–deficient mice (n = 14) were subjected to IVC stenosis. Thrombus prevalence (E), thrombus weight (F), and thrombus length (G) were measured at 48 hours after the surgery. (H) Western blot analysis of clots from WT, caspase-1– and caspase-11–deficient mice. IL-1β in the lysates of clots was measured by immunoblot with an anti–IL-1β polyclonal antibody. NS, not significant .