Figure 3.
RNA-seq analysis identifies ETP-related gene signatures enriched in Kras−/−; NrasQ61R/+ thymocytes. RNA-seq analysis of thymocytes from moribund Kras−/−; NrasQ61R/+ (K−/−; Q/+) mice (n = 3) and age-matched controls (con) (n = 3). (A) Volcano plot of gene expression in K−/−; Q/+ cells vs control T cells. Dots located in the upper-left and upper-right sections represent 969 down- and 506 up-regulated genes respectively (fold-change [FC] >2 and P < .05). (B) Overview of GSEA. FDR, false discovery rate; NES, normalized enrichment score. (C-G) GSEA identified numerous gene signatures enriched in K−/−; Q/+ cells. (C-G) Representative examples include NRAS targets and ERK1/2 cascade (C), stem cell and myeloid cell development (D), IL6/JAK/STAT3 (E), TP53 targets (F), and human ETP-ALL gene signatures (G).

RNA-seq analysis identifies ETP-related gene signatures enriched in Kras−/−; NrasQ61R/+ thymocytes. RNA-seq analysis of thymocytes from moribund Kras−/−; NrasQ61R/+ (K−/−; Q/+) mice (n = 3) and age-matched controls (con) (n = 3). (A) Volcano plot of gene expression in K−/−; Q/+ cells vs control T cells. Dots located in the upper-left and upper-right sections represent 969 down- and 506 up-regulated genes respectively (fold-change [FC] >2 and P < .05). (B) Overview of GSEA. FDR, false discovery rate; NES, normalized enrichment score. (C-G) GSEA identified numerous gene signatures enriched in K−/−; Q/+ cells. (C-G) Representative examples include NRAS targets and ERK1/2 cascade (C), stem cell and myeloid cell development (D), IL6/JAK/STAT3 (E), TP53 targets (F), and human ETP-ALL gene signatures (G).

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