RPS15 mutations rewire CLL translational program. (A) Volcano plot indicating the results from differential TE analysis in RPS15^mut vs RPS15^wt CLL cases. The x-axis represents the log2FC of TE, while the y-axis represents P values. Genes that show statistically significant differential TE (log2FC ≥ |1|; P < .05) are highlighted in light blue. Genes highlighted in red are those implicated in the translation machinery, while genes in dark blue have already been implicated in CLL biology. (B) Supervised hierarchical clustering analysis based on the differentially translated genes between RPS15^mut and RPS15^wt CLL cases. (C) The figure shows enriched pathways from the Reactome database (false discovery rate < 0.05) and the differentially translated genes between RPS15^mut and RPS15^wt CLL cases. Upregulated genes in RPS15^mut compared with RPS15^wt cases are shown in red, while downregulated genes are shown in blue. (D) An example of the enriched immune signaling pathway. The interactions are confirmed by Biogrid. Upregulated genes in RPS15^mut compared with RPS15^wt cases are shown in red, while downregulated genes are shown in green.