Figure 2.
PAR1 and PAR2 activation augments poly(I:C)-induced expression of TF and CXCL8 mRNA. HUVECs and EA.hy926 cells were stimulated with poly(I:C) (12.5 μg/mL) and PAR-specific agonist peptides (150 μM; PAR-1, SFLLRN-NH2 and TFLLRN-NH2; PAR-2, SLIGKV-NH2; PAR-3, TFRGAP-NH2; and PAR-4, GYPGV-NH2) for 3 hours and TF (A,C) and CXCL8 (B,D) mRNA abundance relative to GAPDH were measured by RT-PCR (n = 6-18). Data represent the mean ± standard deviation of the target gene/GAPDH ratio relative to the control. ****P < .0001 by ANOVA followed by a multiple-comparison test. ns, not significant.

PAR1 and PAR2 activation augments poly(I:C)-induced expression of TF and CXCL8 mRNA. HUVECs and EA.hy926 cells were stimulated with poly(I:C) (12.5 μg/mL) and PAR-specific agonist peptides (150 μM; PAR-1, SFLLRN-NH2 and TFLLRN-NH2; PAR-2, SLIGKV-NH2; PAR-3, TFRGAP-NH2; and PAR-4, GYPGV-NH2) for 3 hours and TF (A,C) and CXCL8 (B,D) mRNA abundance relative to GAPDH were measured by RT-PCR (n = 6-18). Data represent the mean ± standard deviation of the target gene/GAPDH ratio relative to the control. ****P < .0001 by ANOVA followed by a multiple-comparison test. ns, not significant.

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