Mutational landscape of nm-PMBLsig⁺ tumors. (A) Oncoplot showing the somatic mutational landscape of nm-PMBLsig⁺ tumors. Genes mutated in at least 4 nm-PMBLsig⁺ tumors (≥27%) and translocations affecting MYC, BCL2, and BCL6 are shown. Colors represent various mutation types, as indicated. (B) Mutational frequency of selected genes in nm-PMBLsig⁺ tumors, GCB DLBCL, and bf-PMBL. Mutational frequencies of genes depicted in panel A are shown for nm-PMBLsig⁺ tumors (BCC DLBCL cohort), GCB DLBCL (Schmitz/Staudt cohort), and bf-PMBL (BCC PMBL cohort). Purple and orange asterisks represent significance of enrichment of mutations in nm-PMBLsig⁺ tumors vs bf-PMBL and nm-PMBLsig⁺ tumors vs GCB DLBCL, respectively (Fisher’s exact test). MYC, BCL2, and BCL6 translocation status was not assessed or has not been reported for the bf-PMBL and DLBCL samples from the Schmitz cohort. (C-E) Mutational enrichment plots showing mutational frequencies per gene in nm-PMBLsig⁺ tumors vs bf-PMBL (C), nm-PMBLsig⁺ tumors vs GCB DLBCL (D), and nm-PMBLsig⁺ vs all DLBCLs (E). Size and opacity of the data points are proportional to the significance of enrichment or depletion of the number of mutations affecting a given gene in nm-PMBLsig⁺ samples compared with bf-PMBL (C), GCB DLBCL (D), or all DLBCL (E) (Fisher’s exact test). FDR, false discovery rate; FS, frameshift.