Characterization of TAMs into murine MCL syngeneic mouse model. Immunocompetent C57BL/6 mice were subcutaneously injected with 5 × 10⁶ FC-muMCL1 murine MCL cell line, and images of syngeneic MCL tumors after 21 days (A) and tumor growth curves (B) of the FC-muMCL1 syngeneic tumors are shown (data are presented as mean ± standard deviation; n = 6 mice/group). (C) Immunofluorescent staining was performed on the MCL mouse syngeneic tumors using F4/80 (red) antibody. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). (D) Immunohistochemistry was performed on the MCL mouse syngeneic tumors by using specific antibodies against CD68, CD80, and CD206. (E) Dot plots of macrophage populations (CD11b⁺/F4/80⁺ and CD80/CD206) with in the MCL mouse syngeneic tumors shown by flow analysis. All staining was performed on at least 2 to 3 mouse tumors, and a representative image is shown. H&E, hematoxylin and eosin.