Expansion and trafficking of CD70-CAR T cells in vivo. (A) The experimental setup. NSG mice underwent AML engraftment with 1 × 106 Molm-13 cells and injection of 5 × 106 CD70-CAR or NT T cells genetically modified to express a luciferase-GFP fusion protein 4 days later. T-cell expansion and trafficking were monitored by BLI on days 4, 7, 11, and 15 (n = 5 for each treatment group). (B) BLI of mice on the indicated days after infusion of CD70-CAR or NT T cells. (C) Quantitative analysis of BLI for each treatment group. Data are the mean ± standard deviation (SD) of 5 animals per treatment group. The area under the curve was calculated for each treatment group and compared by unpaired Student t test; ****P < .0001. (D) The body weight of treated mice was measured as an indicator of their overall condition. The change in body weight compared with the day of T-cell injection for each treatment group is depicted. Data represent the mean of each group ± SD. Results represent pooled data from 5 animals.