The spatial localization of A, AB, B, and DN cells in mouse tibias. (A) A representative image of a whole tibia from a Prrx1:EYFP mouse used for localization studies with the different colors defining the growth plate (red), trabecular bone (yellow), cortical bone (light blue), and border of metaphysis to diaphysis (magenta), the latter defined as being ≥3000 µm from the growth plate. Nuclei (white) highlights the bone marrow cells. (B-D) The proportions of the different cell types are shown for the growth plate (B), metaphysis (C), and diaphysis regions (D). Data are presented as mean ± SD from 4 Prrx1:EYFP mice. (E-H) Shown are the distances of individual cells within the metaphysis region to the growth plate (E), trabecular bone (F), and metaphyseal cortical bone (G) and within the diaphyseal region to the cortical bone (H). Data are presented as median with interquartile range (n = 4 Prrx1:EYFP mice). Gray asterisks directly above each data set are comparisons to random dots, and the asterisks above the bars highlight differences between distinct cell populations. Data were analyzed using 1-way ANOVA with Tukey’s multiple comparisons test (B-D) or Kruskal-Wallis test with Dunn’s multiple comparisons test (E-H). *P < .05; **P < .01; ***P < .001; ****P < .0001.