AB and B cells express osteoblastic genes, whereas A and DN cells express chondrocyte genes. (A-B) Shown are the expression of gene sets described by Baryawno et al²³  for osteoblastic (A) and chondrogenic (B) genes in microarrays of cells of each population. (C-D) Gene set variation analysis (GSVA) for osteoblastic (C) and chondrocyte (D) gene sets. Data represent 4 independent experiments with cells obtained from 4 mice per experiment. (E-J) Gene expression qPCR validation of Runx2 (E), Sp7 (F), Alpl (G), Pth1r (H), Bglap (I), and Sost (J). Data represent 7 independent experiments with cells pooled from 3 to 4 mice. All data are mean ± SD. One-way ANOVA with Tukey’s multiple comparisons test: *P < .05; **P < .01; ***P < .001. TFs, transcription factors.