AB cells exhibit a LepR-MSC gene expression profile. (A-B) Shown are the expression of gene sets described by Baryawno et al²³  for LepR-MSCs (A) and pericyte (B) genes in microarrays of cells from each population. (C-D) GSVA for LepR-MSC (C) and pericyte (D) gene sets (n = 4 independent experiments with cells obtained from 4 mice per experiment). (E-H) Gene expression qPCR validation of Pparg (E), Cebpa (F), Adipoq (G), and Acta2 (H) (n = 7 independent experiments of cells pooled from 3 to 4 mice). (I-L) Shown are the proportions of A, AB, B, and DN cells expressing cell surface LEPR (n = 10; I) and VCAM1 (n = 9; J) and representative FACS plots of LEPR (K) and VCAM1 (L) for each population. All data are mean ± SD. One-way ANOVA with Tukey’s multiple comparisons test: *P < .05; **P < .01; ***P < .001; ****P < .0001.