T-cell response and cytokine changes over time in peripheral blood. (A) Median percentage change from baseline of patient peripheral circulating T-cell subtypes. Dividing T cells were identified by Ki-67⁺, activated T cells by HLA-DR⁺, regulatory T cells by CD4⁺/CD127⁻/CD25^hi/CCR4⁺. Cell counts were derived from a dual platform method of no-wash absolute T-cell quantification and T-cell subtype percentages. Boxes indicate the interquartile range, horizontal white lines indicate the median values, and the bars extend to the upper and lower adjacent values. (B) Average fold change of cytokines over all patients compared with baseline. (C) Change from baseline in levels of IP-10, IL-18, and IFN-γ in 2 treatment parts over time. Boxes indicate the interquartile range, horizontal black lines indicate the median values, and the bars extend to the upper and lower adjacent values. P values for all panels were generated by Student t test. APRIL, a proliferation inducing ligand; BAFF, B-cell–activating factor; BDNF, brain-derived neurotrophic factor; BSLN, baseline; LAP, latency associated peptide; MCP, monocyte chemoattractant protein; MIG, monokine induced by IFN-γ; MIP, macrophage inflammatory protein; MMP, matrix metalloproteinase; MPIF, myeloid progenitor inhibitory factor; sCD30, soluble CD30; SCF, stem cell factor; SDF, stromal cell–derived factor; TGF, transforming growth factor; TNF, tumor necrosis factor.