qRT-PCR analysis. (A) Hepatic hepcidin. (B), Id1. (C) Bmp6 mRNA. Each group consists of at least 3 animals. Data shown are means ± SD. A 2-tailed Student t test was used to analyze the data for 5-week-old mice, and 1-way ANOVA and Tukey’s posttests were used to analyze the data for 8- and 15-week-old mice. *P < .05; **P < .01; ***P < .001.