GADD45g inhibits progression of human AML cells in NOD/SCID mice. (A-B) Molm-13-luc2 cells were transfected with Dox-inducible GADD45g lentiviral vector and engrafted into NOD/SCID mice (8 × 10⁵ cells per mouse). Dox 1 mg/mL was added to drinking water 7 days after transplantation for 1 week to induce GADD45g expression. (A) Bioluminescence imaging of representative mice from each group taken at day 7 and day 25 posttransplantation. (B) Kaplan-Meier survival curves are shown for 2 cohorts of transplanted mice. P values were determined by using the log-rank test (n = 5). NOD/SCID mice were inoculated via the tail vein with 5 × 10⁵ Molm-13 cells with or without GADD45g knockdown. (C) Bioluminescence imaging of representative mice from each group taken at day 25 posttransplantation. (D) Kaplan-Meier survival curves are shown for 2 cohorts of transplanted mice. P values were determined by using the log-rank test (n = 5). (E) NOG mice were transplanted with 1 × 10⁶ human CB CD34⁺ cells transduced with lentiviruses containing GADD45g or control vector. Percentages of hCD45⁺GFP⁺ cells in the BM of NOG mice at 16 weeks’ posttransplantation. Data are represented as the mean ± SD; n = 5 mice per group. (F) The percentage of CD19⁺ and CD33⁺ cells within the GFP⁺CD45⁺ population in the BM of NOG-recipient mice at 16 weeks’ posttransplantation. (G) The percentage of CD235a⁺ cells within the GFP⁺CD45^– population in the BM of NOG-recipient mice at 16 weeks’ posttransplantation. Data are presented as the mean ± SD, and were analyzed by using the 2-tailed Student t test. *P < .05, **P < .01. NS, not significant; shCtrl, scramble small hairpin RNA; shGG, GADD45g small hairpin RNA.