Neutrophildevelopment is normal in mice with myeloid norbin deficiency. (A) Schematic showing the generation of mouse strains with myeloid norbin deficiency (Ncdn^Δmye) and with combined norbin/Prex deficiency (Ncdn^ΔmyePrex1/2^−/−). Norbin deletion from resident peritoneal leukocytes and LPS-induced peritonitis leukocytes (B) and bone marrow–derived isolated neutrophils (C) of Ncdn^Δmye mice. Norbin western blots (WBs) and Coomassie staining of total cell lysates. (D) Quantification of mature bone marrow–derived neutrophils of the indicated genotypes. Data are mean ± standard error of the mean of 10 independent experiments. (E) Quantification of Ly6G^hi, CD11b^hi neutrophils in peripheral blood as percentage of total leukocytes. Data are mean ± standard error of the mean; dots represent individual mice. (F) Representative flow cytometry plots showing expected numbers and marker protein levels of mature CD11b^hi, Ly6G^hi neutrophils in bone marrow as percentage of total leukocytes. (G) Representative cytospin images (Diff-Quik stain) showing the characteristic nuclear morphology of mature isolated neutrophils as in panel D.