p-proteome analyses reveal distinct phosphorylation patterns in UM-CLL and M-CLL. (A) Number of differentially regulated p-peptides in untreated UM-CLL vs M-CLL samples. (B) Volcano plot of basal protein phosphorylation in untreated UM-CLL cells vs M-CLL. (C) pS:pT:pY ratio of all isolated p-peptides in untreated UM-CLL cells vs M-CLL. (D) pS:pT:pY ratio of all differentially regulated p-peptides in untreated UM-CLL cells vs M-CLL. (E) Heat map representing basic phosphorylation levels of the most relevant regulated proteins in untreated M-CLL and UM-CLL cells (cell migration and motility). (F) pS:pT:pY ratios in M-CLL and UM-CLL upon BCR stimulation compared with native cells (left columns), ibrutinib treatment compared with native (nat.) cells (middle columns), and a combination of BCR stimulation and ibrutinib treatment compared with BCR stimulation alone (right columns). (G) Number of differentially regulated p-peptides in UM-CLL and M-CLL upon BCR stimulation compared with native, with corresponding volcano plot. (H) Number of differentially regulated p-peptides in UM-CLL and M-CLL upon ibrutinib treatment compared with native cells and corresponding volcano plot. (I) Number of differentially regulated p-peptides upon ibrutinib treatment plus BCR stimulation compared with BCR stimulation only with corresponding volcano plot.