Robust ADA2 expression, secretion, and enzymatic activity in CD34-derived cells after LV-ADA2 transduction. ADA2 expression was measured at transcript (A) and protein (B) levels in expanded CD34-derived cells from HDs that were left UT or transduced with the LV-ADA2 and LV-GFP at an MOI of 10, 50, and 100. GAPDH was included as a loading control. Levels of secreted ADA2 are also shown. ADA2 protein (C) and enzymatic activity (D) were measured in cell-free supernatants collected from cells transduced with LV-ADA2 and LV-GFP at an MOI of 10, 50, and 100. Data are mean ± standard deviation. *P < .05.