iNKT1, but not iNKT2 and iNKT17, cells display cytotoxic potential. (A) Genome tracks showing a comparison of ATAC-seq and RNA-seq profiles for genes encoding cytotoxic molecules in iNKT1 (blue), iNKT2 (red), and iNKT17 (green) cells. Data are merged from 2 biological replicates. Black boxes highlight differentially accessible ATAC-seq peaks. (B) In vitro cytotoxicity by iNKT sublineages against α-galactosylceramide (αGalCer)-loaded CD1d-transduced A20 lymphoma (A20-CD1d) cells after 24 hours of coculture (effector:target ratio of 4:1). Represented data are pooled from 3 independent experiments performed in triplicate. (C) Survival after A20-CD1d (2 × 10e4) cells intravenously injected into sublethally irradiated (4.4 Gy) wild-type BALB/c mice treated with iNKT cell subsets (5 × 10e4 cells; iNKT1, blue; iNKT2, red; iNKT17, green) or untreated (black). Results are pooled from 2 independent experiments with a total of 10 mice per group. Survival curves were plotted by using the Kaplan-Meier method and compared by using a log-rank test. P values are indicated when significant.