Adoptive transfer of distinct iNKT subsets differentially affect CD4 and CD8 Tcon transcriptome during murine acute GVHD. RNA-seq analysis of CD4 (A,C) and CD8 (B,D) Tcon recovered at day 7 post-HCT from animals treated with the indicated iNKT sublineages (untreated, gray; iNKT1, blue; iNKT2, red; iNKT17, green). (A-B) Principal component analysis of transcriptome based on the top 500 differentially expressed genes across all samples. (C-D) Heatmap and hierarchical clustering based on the 500 most highly differentially expressed genes across all samples. Immune-related genes are highlighted. Expression for each gene is scaled (z scored) across single rows. Analysis was performed on 3 biological replicates from 2 independent experiments.