iNKT2 and iNKT17 cells inhibit IL-18Rα and CD49d expression and prevent tissue damage during murine acute GVHD. IL-18Rα (A) and CD49d (B) expression at the surface of CD4 (upper panels) and CD8 (lower panels) Tcon recovered at day 7 after HCT from spleen of mice treated with different iNKT sublineages (untreated, gray; iNKT1, blue; iNKT2, red; iNKT17, green). Representative FACS histograms (left panels) and summary bar plots (right panels) are shown. Data are pooled from 2 independent experiments with 3 to 5 mice per group. (C) Representative photomicrographs of hematoxylin and eosin–stained sections of small intestine and colon (×200) collected 7 days after transplantation from indicated groups. GVHD tissue damage manifests as inflammation (open arrow), crypt apoptosis (solid arrow), and crypt loss (solid asterisk). Histopathologic GVHD score (consisting of scores for crypt apoptosis and inflammation) for small intestine and colon for the indicated groups. Data are pooled from 2 independent experiments with a total of 6 mice per group. P values are indicated when significant.