In silico predicted adenoviral epitopes elicit a VST response by intracellular flow cytometry. (A) Representative example of VST product was stimulated with both the entire adenoviral Pepmix and single peptides synthesized on the basis of epitope prediction. Each single peptide is predicted to be presented by the noted HLA molecule present within the cellular product. Differential degree of IFN-γ positivity seen by intracellular flow cytometry, indicating that multiple epitopes are involved in provoking a T-cell response in this product. CD4-predominant response is seen, but little CD8 response is observed.