DPT reduces HSPC homing in the zebrafish. (A) Schema of zebrafish homing assays. Zebrafish received 25-Gy radiation and 2 days later underwent HCT via intracardiac injection of 250 000 ubi:luciferase or bactin2:EGFP donor WKM cells. (B) Representative BLI 48 hours after transplantation of ubi:luciferase marrow cells pretreated with rDPT (or bovine serum albumin) (left panel). BLI was determined using an IVIS Spectrum in vivo imaging system. The recipient WKM region of interest is circled in red. Bar graph indicating BLI signal quantification is shown (right panel). (C) Flow cytometry quantification of homed of bactin2:EGFP donor marrow cells 16 hours after HCT. n = 20 or 21 animals per group in 3 independent experiments. All data are means with standard deviations. The P values were derived using the Student t test. BSA, bovine serum albumen; +/−, with or without.