Figure 1.
Gene expression in unsorted cells and LPCs. (A) Gene expression of CD19, CD58, CD97, and CD200 in BM samples from 5 BCP-ALL patients (1, 12, 23, 61, 62) and from 5 healthy donors were analyzed using Agilent Whole Genome Oligo microarrays. Data show side-by-side comparisons of median centered normalized log2 signal intensities. (B) BCP-ALL patients were sorted on the basis of expression or lack of expression of CD34 and CD19. CD34+/CD38– HSCs were sorted from normal BM MNCs before gene expression analyses. Bars represent median normalized log2 intensities of expression, and error bars represent the interquartile ranges. Results were analyzed by 2-way analysis of variance (ANOVA) with Tukey’s post hoc testing. *P ≤ .05; **P ≤ .01; ***P ≤ .001.