Effects of IgM or MyD88 targeting on growth of TP53/CDKN2A/2B-ko cells. (A) Time-course analysis of changes in IgM mutant vs wild-type allele ratio and surface IgM expression in TCL1-863 TP53/CDKN2A/2B/MGA-ko cells. The presence of 2 bands corresponding to the wild-type IgM heavy chain constant region gene (red arrows) is a result of the occasional nontemplated addition of a nucleotide at the 3′ end of PCR-amplified products by the Taq DNA polymerase.⁶⁰ (B) Changes in IgM mutant vs wild-type allele ratio in TCL1-355 TP53/CDKN2A/2B-ko cells grown in vitro or in vivo. Cells were transfected with the RNP complex targeting the IgM heavy chain constant region gene and placed in culture. After 48 hours, cells were split and continued to be grown in vitro or were injected in the peritoneal cavity of an NSG mouse that was euthanized 28 days later. One of 2 experiments with identical results is shown. (C) Time-course analysis of changes in MyD88 mutant vs wild-type allele ratio in TCL1-355 TP53/CDKN2A/2B-ko cells. (D) BrdU-incorporation analysis of TCL1-355 TP53/CDKN2A/2B-ko cells with wild-type or mutated MyD88. Analysis was performed on cells collected 21 days after transfection with the MyD88 or control RNP complex.