Effects of BCR inhibitors and palbociclib on the proliferation of human RS cells. (A) Effects of BCR inhibitors on spontaneous proliferation of RS9737 cells after 48 hours in culture. All inhibitors were used at 1 μM concentration. Three independent experiments, each with 3 technical replicates, were performed. Statistical analysis was performed using 1-way repeated measures ANOVA with the post hoc Tukey test. (B) Effects of palbociclib (1 μM) on spontaneous proliferation of RS9737 cells after 48 hours in culture. Statistical analysis was performed using the paired Student t test. (C) Analysis of activity of R406 and palbociclib, alone or in combination, against unstimulated and CpG plus IL15 or CD40L plus IL4 plus IL21 stimulated RS9737 cells. (D) Analysis of CI for R406 plus palbociclib against RS9737 cells. The CI was calculated as described in Figure 6D.