Activation profiles of SASH3-mutated T cells after in vitro stimulation with mitogens. (A) Representative plots of CD25, CD71, CD98, and GLUT1 expression in (left) CD4⁺ and (right) CD8⁺ T cells from P2 (red) and a healthy control (solid gray) in resting conditions or upon activation with anti-CD3 and anti-CD28 or anti-CD3 and anti-CD28 plus IL-2 in CD4⁺ T cells. (B) Cumulative mean fluorescence intensity (MFI) data for CD25, CD71, CD98, and GLUT1 expression in (left) CD4⁺ and (right) CD8⁺ T cells from controls (n = 5; solid gray) and SASH3-mutated patients (red). Bars represent mean values ± standard error of the mean. *P ≤ .05. Unstim, unstimulated.