Increased Gata2 expression in preleukemic mouse myeloid cells expressing PML-RARA. (A) Heat map of the 3361 DEGs between lineage-depleted and flow-purified GFP⁺ WT mouse bone marrow cells 7 days following retroviral transduction with PML-RARA^WT compared with those transduced with an empty MSCV-IRES-GFP vector control by bulk RNA-seq (fold change ≥2 and FDR <0.05). Also plotted is the expression of the same DEGs in cells transduced with PML-RARA^C88A MSCV-IRES-GFP vector. (B) Volcano plot of the expression changes in (A). Upregulated (red) and downregulated (blue) DEGs (fold change ≥2 and FDR <0.05) are highlighted. Expression changes in Bcl2, Zbtb16 (Plzf1), Hgf, Pdgfrb, Hdc, Elane, Prtn3, and Cd177 are labeled. (C) Relative Gata2 RNA expression in lineage-depleted and flow-purified GFP⁺ WT mouse bone marrow cells 7 days following retroviral transduction with PML-RARA^WT, PML-RARA^C88A, or an empty MSCV-IRES-GFP vector. Data in panels A-C are from 3 independent biological replicates, each from a separate 6- to 8-week-old WT mouse. (D) Heat map of the 1838 DEGs between flow-purified promyelocytes from preleukemic Ctsg-PML-RARA mice compared with those from WT littermates by bulk RNA-seq (fold change ≥2 and FDR <0.05). (E) Volcano plot of the expression changes in (D). Upregulated (red) and downregulated (blue) DEGs (fold change ≥2 and FDR <0.05) are highlighted. Expression changes in Bcl2, Zbtb16 (Plzf1), Hgf, Pdgfrb, Hdc, Elane, Prtn3, and Cd177 are labeled. (F) Relative Gata2 RNA expression in flow-purified promyelocytes from preleukemic Ctsg-PML-RARA mice compared with those from WT mice by RNA-seq. Data in panels D-F are from 3 pairs of mice that were littermate matched and 6 to 8 weeks of age; each flow-purified promyelocyte sample was from a unique mouse. P values are corrected for multiple testing. ****P < .0001.