Somatic mutations identified by NGS profiling in patients with CIN. (A) The graph shows the frequency of somatic mutations identified in the patients. A total of 25 somatic mutations were detected in 21 patients: 12 mutations were detected in DNMT3A and 7 mutations in TET2, followed by 3 mutations in IDH1/IDH2, 2 mutations in SRSF2, and 1 mutation in ZRSR2. The bars show the number of mutations per gene. (B) The bars show the number of patients harboring 1 to 3 mutations in the aforementioned genes. The majority of patients (n = 18) harbored 1 mutation, 2 patients were detected with 2 mutations (in 2 genes [IDH2/SRSF2] and 1 gene [TET2], respectively) and 1 patient harbored 3 mutations (1 in IDH1 and 2 in DNMT3A). (C) The graph shows the base-pair changes in the somatic variants. The bars depict the proportion of variants with a single nucleotide substitution. A proportion of 31.8% of the single-nucleotide sequence corresponded to a C > T substitution.