Figure 1.
Single-cell transcriptomes of primary murine MKs spanning each ploidy stage. (A) Schematic illustration of the experimental workflow. Individual MKs at different DNA content levels from 2N to 32N were collected from mouse BM by FACS and manual picking and were processed for scRNA-seq. Fluorescence in situ hybridization (FISH) was used to verify the DNA contents of the sorted MKs. (B) FACS results for mouse MKs that span each ploidy stage (2N-32N) stained with CD41 and Hoechst 33342 antibodies. (C) Representative images of MKs analyzed by DNA-FISH to detect the degree of ploidy and to quantify the accuracy of ploidy degree and cell numbers of MKs in each ploidy. Scale bars, 10 μm. (D) Violin plots showing relative expression levels of traditional markers of MKs in different ploidy MKs and CD41– cells. APC, antigen-presenting cell; SSC, side scatter.

Single-cell transcriptomes of primary murine MKs spanning each ploidy stage. (A) Schematic illustration of the experimental workflow. Individual MKs at different DNA content levels from 2N to 32N were collected from mouse BM by FACS and manual picking and were processed for scRNA-seq. Fluorescence in situ hybridization (FISH) was used to verify the DNA contents of the sorted MKs. (B) FACS results for mouse MKs that span each ploidy stage (2N-32N) stained with CD41 and Hoechst 33342 antibodies. (C) Representative images of MKs analyzed by DNA-FISH to detect the degree of ploidy and to quantify the accuracy of ploidy degree and cell numbers of MKs in each ploidy. Scale bars, 10 μm. (D) Violin plots showing relative expression levels of traditional markers of MKs in different ploidy MKs and CD41– cells. APC, antigen-presenting cell; SSC, side scatter.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal