Figure 1.
Abnormal BM composition in TIMP-compromised mice. (A) Breeding strategy to generate QT3+/− and QT mice. (B) Growth curves of: left, young WT (n = 12), QT3+/+ (n = 7), QT3+/− (n = 6), and QT mice (n = 5); middle, adult male WT (n = 6), QT3+/+ (n = 12), QT3+/− (n = 12), and QT (n = 4) mice; and right, adult female WT (n = 10), QT3+/+ (n = 10), QT3+/− (n = 11), and QT (n = 2) mice. QT pups are indistinguishable from their littermates at birth but show growth restriction by 5 days of age. Male and female QT mice are runted and have shortened lifespans. Data points are the mean plus or minus standard error of the mean (SEM). Statistics by 1-way analysis of variance (ANOVA) with *P < .05. (C) Representative image of WT and QT mice at 7 weeks of age. (D) Sagittal tibiae sections from 4-week-old mice (top panel) and sagittal femoral sections from 7-week-old mice (bottom panel) stained with hematoxylin and eosin (H&E); scale bar, 500 μm. (E) Isosurface rendering of femurs from 7-week-old mice using high-resolution micro–computed tomography imaging highlights the partial loss of trabecular bone in QT3+/− mice and complete loss in QT mice. Top panels, transverse view; middle and bottom panels, planer views at different depth. (F) Percentage (left) and total number (right) of live B-cell (B220+) in WT (percentage, n = 21; cell number, n = 15), QT3+/− (percentage, n = 18; cell number, n = 13), and QT (percentage, n = 13; cell number, n = 7) BM from 4-week-old littermates. Statistics by 1-way ANOVA; individual data points are shown as well as the mean plus or minus SEM with **P < .01, and ***P < .001. (G) Percentage (left) and total number (right) of CD11b+Gr-1+ myeloid cells in WT (n = 13), QT3+/− (n = 11), and QT (n = 8) BM from 4-week-old mice. Statistics by 1-way ANOVA; data represent mean plus or minus SEM with ***P < .001. (H) Percentage and total pro-B-cell in BM of 4- to 5-week old WT (n = 11) and QT3+/− (n = 10) mice. Statistics by the Student t test; data represent mean plus or minus SEM with *P < .05. (I) Percentage and total pre-B-cell in BM of 4- to 5-week old WT (n = 11) and QT3+/− (n = 10) mice. Statistics by the Student t test; data represent mean plus or minus SEM with *P < .05.

Abnormal BM composition in TIMP-compromised mice. (A) Breeding strategy to generate QT3+/− and QT mice. (B) Growth curves of: left, young WT (n = 12), QT3+/+ (n = 7), QT3+/− (n = 6), and QT mice (n = 5); middle, adult male WT (n = 6), QT3+/+ (n = 12), QT3+/− (n = 12), and QT (n = 4) mice; and right, adult female WT (n = 10), QT3+/+ (n = 10), QT3+/− (n = 11), and QT (n = 2) mice. QT pups are indistinguishable from their littermates at birth but show growth restriction by 5 days of age. Male and female QT mice are runted and have shortened lifespans. Data points are the mean plus or minus standard error of the mean (SEM). Statistics by 1-way analysis of variance (ANOVA) with *P < .05. (C) Representative image of WT and QT mice at 7 weeks of age. (D) Sagittal tibiae sections from 4-week-old mice (top panel) and sagittal femoral sections from 7-week-old mice (bottom panel) stained with hematoxylin and eosin (H&E); scale bar, 500 μm. (E) Isosurface rendering of femurs from 7-week-old mice using high-resolution micro–computed tomography imaging highlights the partial loss of trabecular bone in QT3+/− mice and complete loss in QT mice. Top panels, transverse view; middle and bottom panels, planer views at different depth. (F) Percentage (left) and total number (right) of live B-cell (B220+) in WT (percentage, n = 21; cell number, n = 15), QT3+/− (percentage, n = 18; cell number, n = 13), and QT (percentage, n = 13; cell number, n = 7) BM from 4-week-old littermates. Statistics by 1-way ANOVA; individual data points are shown as well as the mean plus or minus SEM with **P < .01, and ***P < .001. (G) Percentage (left) and total number (right) of CD11b+Gr-1+ myeloid cells in WT (n = 13), QT3+/− (n = 11), and QT (n = 8) BM from 4-week-old mice. Statistics by 1-way ANOVA; data represent mean plus or minus SEM with ***P < .001. (H) Percentage and total pro-B-cell in BM of 4- to 5-week old WT (n = 11) and QT3+/− (n = 10) mice. Statistics by the Student t test; data represent mean plus or minus SEM with *P < .05. (I) Percentage and total pre-B-cell in BM of 4- to 5-week old WT (n = 11) and QT3+/− (n = 10) mice. Statistics by the Student t test; data represent mean plus or minus SEM with *P < .05.

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