Altered gene-expression profile of TIMPless LSK cells to reduce B lymphopoiesis. (A) Schematic of experimental pipeline for microarray. (B) Volcano plot for differential messenger RNA (mRNA) abundance levels in the 4 cell compartments. x-axis (fold change [FC]), Coefficients from linear model in log2 scale; y-axis, P values adjusted for multiple testing. The dotted line depicts q value < 0.25. Genes with q value < 0.25 and absolute log₂ [FC] > 2 are labeled directly on the graphs. (C) Venn diagram of significantly altered genes (q value < 0.25). (D) Heat map display of significantly altered B-cell–related genes in LSK cells. Asterisks mark transcription factors (WT, n = 3; QT, n = 3). (E) G:profiler pathway analysis visualized by Cytoscape using significant (q value < 0.25) genes in the LSK compartment. (F) mRNA levels of 3 candidate B-cell–related genes in WT (n = 3) and QT (n = 3) LSK cells, relative to glucuronidase β (GusB) control mRNA, by ddPCR. Data represented as mean plus or minus SEM; statistics by the 2-tailed unpaired Student t test; **P < .01, and ***P < .001. FSC-A, forward scatter area; SSC-A, side scatter area.