IK6 knock-in shows changes in gene expression patterns similar to heterozygous knockout. All samples are from Nalm-6 clonal cell lines. (A) Heatmap showing differential gene expression analysis from RNAseq from WT, heterozygous (HET), KO, and IK6 knock-in. (B) Volcano plot highlighting the most significantly upregulated or downregulated genes (IK6 vs WT). Genes highlighted in blue are included in confirmatory follow-up. (C) Flow cytometry histograms showing confirmation of protein expression changes of a panel of cell surface markers commonly used in leukemia phenotyping. MFI, mean fluorescence intensity.