Platelet activation by Low Fuc High Gal IgG1 immune complexes is dependent on FcγRIIA and functions at low and high shear. (Ai) Volume of thrombi formed on VWF plus immune complexes containing spike and either WT IgG or IgG with Low Fuc High Gal in the presence or absence of 20 μg/mL IV.3 and (Aii) representative images (acquired at ×20 original magnification) of thrombi stained with DiOC₆. (Bi) Volume of thrombi formed on VWF plus WT IgG or IgG with Low Fuc High Gal at a shear rate of 200 s⁻¹ or 1000 s⁻¹ and (Bii) representative images of thrombi (acquired at ×20 original magnification) stained with DiOC₆. Values are mean ± SEM. Significant differences were tested by 2-way ANOVA with the Tukey multiple comparisons test. *P < .05; **P < .01. Veh, vehicle.