A densely interconnected heptad autoregulatory circuit persists in AML cells with altered connectivity compared with CD34⁺ HSPCs. (A) ChIPseq binding pattern at heptad regulatory regions in CD34⁺ HSPCs (left). Gray boxes indicate regulatory regions not computationally called as binding peaks for the indicated TF. Plots are scaled to 5 times the height of the smallest called peak for that TF to allow visualization of a wide range of peak heights. Corresponding inferred heptad autoregulatory circuit (right). Most regulatory elements have all 7 heptad TFs bound; asterisk and bold border indicate regions where binding of a particular TF is absent. (B) ChIPseq binding pattern at heptad regulatory regions in ME-1 AML cells (left). Gray boxes indicate regulatory regions not computationally called as binding peaks for the indicated TF. Plots are scaled to 5 times the height of the smallest called peak for that TF to allow visualization of a wide range of peak heights. Corresponding inferred heptad autoregulatory circuit (right). Most regulatory elements have all 7 heptad TFs bound; asterisk and bold border indicate regions where binding of a particular TF is absent. (C) ChIPseq binding pattern at heptad regulatory regions in KG-1 AML cells (left). Gray boxes indicate regulatory regions not computationally called as binding peaks for the indicated TF. Plots are scaled to 5 times the height of the smallest called peak for that TF to allow visualization of a wide range of peak heights. Corresponding inferred heptad autoregulatory circuit (right). Most regulatory elements have all 7 heptad TFs bound; asterisk and bold border indicate regions where binding of a particular TF is absent.