RUNX1-ETO induces a T-like chromosomal conformation. (A) 3C sequencing (Hi-C) in Jurkat (upper half) and HL-60 (lower half) cell lines. Black dots indicate the chromosomal looping. (B) Schematic representation of 3C for proximity quantification of indicated elements. PU.1 locus 3C and transcript expression in Jurkat (C) and HL-60 (D) cells (3C, n = 8; RT-qPCR, n = 3). (E) Model of competing sense/AsPrs. (F) Aligned reads of RUNX1-ETO ChIP-seq (antibody against the ETO moiety) in Kasumi-1 cells after small interfering RNA knockdown of RUNX1-ETO (siRUNX-ETO) and mismatch control (siControl). (G) 3C in Kasumi-1 after siRUNX1-ETO (siRE) and siControl (siCtrl) (n = 3). (H) Transcript quantification in Kasumi-1 after siRUNX1-ETO and siControl (n = 8) of RUNX1-ETO (green), PU.1 asRNA, and PU.1 mRNA. (I) Model of RUNX1-ETO inducing a T-lymphoid chromosomal conformation. Data are represented as mean ± standard error of the mean. *P < .05, ***P < .001, Wilcoxon and Student’s t tests.