Expression profiles of LOUP and PU.1 in normal tissues and cell lineages. (A-B) Transcript profiles of LOUP and PU.1 in human tissues. Shown are transcript counts from the Illumina Body Map RNA-seq data set (ArrayExpress [AE]: E-MTAB-513). Error bars indicate SD (n = 2). (C) The proportion of cell lineages corresponding to transcript levels of LOUP and PU.1. scRNA-seq data of human mononuclear cells were retrieved from the 10x Genomic Project.⁵⁴ Myeloid includes monocytes, macrophages, and granulocytes. Numbers on each bar indicate the percentage of each cell type within LOUP⁺/PU.1⁺ (left panel) and LOUP⁻/PU.1⁻ groups (right panel), respectively. (D-E) qRT-PCR analysis of Loup RNA and Pu.1 mRNA levels in murine hematopoietic stem, progenitor, and mature (myeloid) cell populations. Data are shown relative to LT-HSCs. Error bars indicate SD (n = 2). See also supplemental Figure 3 and supplemental Table 3. B, B lymphocyte; CMP, common myeloid progenitor; DC, dendritic cell; Ery, erythrocyte; GMP, granulocyte-macrophage progenitor, myeloid cells (Mac1⁺Gr1⁺); LMPP, lymphoid-primed multipotent progenitor; LT-HSC, long-term hematopoietic stem cell; Meg, megakaryocyte; MEP, megakaryocyte-erythroid progenitor; NK, natural killer cell; Plas, plasma cell; ST-HSC, short-term hematopoietic stem cell; T_CD4+, T helper cell; T_CD8+, cytotoxic T cell; T_reg, regulatory T cell.