Small-molecule screening for modifiers of anemia in Rps14 deficiency identifies imiquimod. (A) The screening design. (B-E) Rps14 4-dpf morphants and controls treated with DMSO, l-leucine, or imiquimod were stained for hemoglobin with o-dianisidine. Ventral views of the head (top) and lateral (bottom) views, with the anterior facing left and the dorsal facing upward. (F) Semiquantitative analysis of the effects of imiquimod on the severity of hemoglobinization. Imiquimod improved the level of hemoglobinization compared with the DMSO control. Statistical comparisons performed by Fisher’s exact test. Original maginfication x80 for panels B-E.