PPAnalysis workflow. A summary of PPAnalysis assay steps (AS) and data steps (DS). AS1, PPAnalysis plate is prepared, freeze-dried, and vacuum sealed. AS2, Flow cytometry assay is performed using platelet-rich plasma. DS1, Concentration-response curves are fitted to data, and sensitivity and response capacity metrics are collected. DS2, Data are summarized. DS3, Data are normalized, and barcodes are produced. Postanalysis hierarchical clustering and PCA are used to identify platelet function phenotypes. The heat map depicts platelet metrics in the rows and individual donor responses in the columns (red indicates high; blue indicates low). Numbers above the heat map correspond to the phenotypic groups identified by clustering.