Characterization of platelet function with PPAnalysis. (A) Examples of concentration-response curves fitted to the data in DS1. Errors in the fitting process are marked “Dirty” (eg, fibrinogen response to epinephrine). (B) Diagram depicting how the summary statistics sensitivity and capacity are extracted from concentration-response curves fitted to data via the Hill function. (C) Examples of donor barcodes that capture a donor’s relative sensitivity and capacity and are produced in data step 3 (DS3). D035 is a donor with high sensitivity to agonists but low capacity, whereas donor D006 has platelets with low sensitivity but a higher capacity, particularly P-selectin exposure in response to CRP and TRAP-6. (D) Variability in measures of capacity and sensitivity for fibrinogen binding and P-selectin exposure in response to the 5 agonists. Outputs from DS1, DS2, and DS3 for all donors are available in the code and data download.